pgel1020  (Addgene inc)

Journal: Microbiology Spectrum

Article Title: Transformation of Gardnerella vaginalis with a Bifidobacterium-Escherichia coli shuttle vector plasmid

doi: 10.1128/spectrum.00481-25

Figure Lengend Snippet: (A ) Growth curves of Gardnerella isolates at 37 ˚C in 7% CO 2 for 30 h. Each point represents an average of three replicates, and error bars indicate standard deviation. ( B ) Restriction activity assay results. “L” Size markers; Lane 1, undigested pKO403- lacZ′- sp; Lane 2, pKO403- lacZ′- sp treated with the supernatant from G. vaginalis ATCC 14018; Lane 3, pKO403- lacZ′- Sp treated with the supernatant from G. vaginalis ATCC 49145.

Article Snippet: Plasmid pKO403- lacZ′- Sp was purchased from Addgene (Cat# 174726) ( ).

Techniques: Standard Deviation, Activity Assay

Journal: Microbiology Spectrum

Article Title: Transformation of Gardnerella vaginalis with a Bifidobacterium-Escherichia coli shuttle vector plasmid

doi: 10.1128/spectrum.00481-25

Figure Lengend Snippet: Spectinomycin tolerance of G. vaginalis ATCC 49145 and G. vaginalis ATCC 49145 with the pKO403- lacZ′- Sp plasmid in agar and broth ^b

Article Snippet: Plasmid pKO403- lacZ′- Sp was purchased from Addgene (Cat# 174726) ( ).

Techniques: Plasmid Preparation, Modification

Journal: Microbiology Spectrum

Article Title: Transformation of Gardnerella vaginalis with a Bifidobacterium-Escherichia coli shuttle vector plasmid

doi: 10.1128/spectrum.00481-25

Figure Lengend Snippet: (A) G. vaginalis grown in the presence of X-gal on MHA supplemented with horse serum. ( B ) G. vaginalis + pKO403- lacZ′- Sp in the presence of X-gal on MHA supplemented with horse serum and 50 µg/mL of spectinomycin. The inset in the top right corner of each panel shows an enlarged single colony. Image taken from Canon R6 with Laowa 100 mm f/2.8 2 × Ultra Macro Apo lens.

Article Snippet: Plasmid pKO403- lacZ′- Sp was purchased from Addgene (Cat# 174726) ( ).

Techniques:

Journal: Microbiology Spectrum

Article Title: Transformation of Gardnerella vaginalis with a Bifidobacterium-Escherichia coli shuttle vector plasmid

doi: 10.1128/spectrum.00481-25

Figure Lengend Snippet: Plasmid stability. G. vaginalis with pKO403- lacZ′- Sp was grown in the presence or absence of spectinomycin (as indicated in the legend) for 18 h or 24 h and then plated in CBA with and without spectinomycin. Colony-forming units on selective and non-selective media were counted to calculate the proportion of Sp R bacteria. Each bar represents the average of three replicates, and error bars indicate the standard deviation.

Article Snippet: Plasmid pKO403- lacZ′- Sp was purchased from Addgene (Cat# 174726) ( ).

Techniques: Plasmid Preparation, Bacteria, Standard Deviation